| Summary: | 碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 93 === Allergic diseases have become one of the modern diseases of civilization today. According to the 2005 report of Y Furuta, there has been a 40% increase in the number of allergic diseases of children around the world. Recent studies has confirmed that in addition to the environmental factor,genetic have an inseparable relationship. In this study, we discussed the many genetic types of allergic diseases that particularly affect Taiwanese children.We choose two candidate allergic gene CC16 and NOS1, to develop three experiments : to access the genotype among families with allergic disease, to compare the genotype of normal healthy children to allergic disease children, and to analyze the relation between single genotype of allergic disease and clinical serum immunoglobulin E, eosinophil count, specific immunoglobulin E antigen, and lung function examination. We apply the polymerase chain reaction(PCR), restriction fragment length polymorphism(RFLP), and sequencing. According to the results of the experiment, it confirmed there is no relationship between the CC16 and NOS1 gene polymorphism with allergic disease Taiwanese elementary school children.
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