The UB2BOS Cell Reaction of Primary Tooth Pulpectomy Materials

• Main Authors: Chung-Wen, 陳重文
• Other Authors: Chia-Tze Kao
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/96598412727998714250

碩士 === 中山醫學大學 === 口腔醫學研究所 === 93 === The most popular root canal filling materials for primary teeth are zinc oxide eugenol, iodoform paste, and calcium hydroxide. It is required that root canal filling materials should be biocompatible with the periapical tissue. There are less papers which indicate the mechanism of cellular change of root canal filling materials. The purpose of the present study was to evaluate the cellular changes of ZOE with FC and Vitapex treated human osteosarcoma cell line. The ZOE powder mixed with the formocresol liquid and Vitapex alone were eluted in culture medium. The elution solution was treated on the human osteosarcoma cell line. The MTT assay was used to investigate the survival rate of the UB2BOS cells. The western blot assay was used to compare the JNK kinase expression. The one way ANOVA statistical method was to compare the survival difference with p<0.05 showed difference. The result showed that ZOE sealer is toxic to UB2BOS cells (p<0.05). The Vitapex is biocompatible with the UB2BOS cells. The JNK kinase expression is seen in ZOE with FC group only. It is concluded that the ZOE with FC is not biocompatible with UB2BOS and the cell death is by apoptosis. Clinically, we suggested that Vitapex is a good choice in primary tooth root canal filling.