Studies on the antioxidant effect of (polyhydroxy-benzylidene)-(hydroxy-phenyl)amines

• Main Authors: Hsiao-Ching Lin, 林小菁
• Other Authors: Chau-Chen Yang
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/57097823436056331855

碩士 === 嘉南藥理科技大學 === 化妝品科技研究所 === 93 === Abstract In this study we aim to originally systhesize a series of highly productive (polyhydroxy-benzylidene)-(hydroxyl-phenyl)amines and observe their antioxidant capacity estimated in four different systems. 1. The effect on DPPH radical savenging 2. The effect on trolox equivalent antioxidant capacity 3. The effect on scavenge nitric oxide. 4. The effect on liposome assay. The results of the study shows : the estimations of both the capture of DPPH and TEAC assay are similar, as the indicator in the two systems are the reduction of free radicals. The results show that A ring has obvious influence on the type, number and position of substituent. However, B ring is decisive only in the positions of substituents and have little to do with the types and existence of substituent. As a whole, A ring has greater effect than B ring, and substituents at the ortho and para position are best effective on antioxidant: DPPH assay IC 50 (2.90~3.40ppm) is 2.5 times better than trolox(7.5~8ppm), TEAC assay IC 50 (0.91~1.02ppm) is about 5-6 times better than trolox(5.56ppm). In liposome and NO assay systems, they are found that in low density of A ring monohydroxyl substituent at A ring are better than dihydroxy or trihydroxy substituents. But, in higher density the suppressive effect in these three composites are about the same. However, the more hydroxy groups of benzylidene phenyl amines are methylated to be methoxy groups at A ring, while the less effect on the antioxidant capacity. But, the methoxy group or not substituent at B ring are not poor effects than hydroxy groups at B ring. This results indicate that the type, number and positions of subtituents, in benzylidene-phenyl amines to play a critical role in exerting the antioxidant