| Summary: | 碩士 === 嘉南藥理科技大學 === 生物科技系暨研究所 === 93 === The prevalence rates of Extended-Spectrumβ-Lactamase-producing Klebsiella pneumoniae（ESBL-KP）among all nosocomial K. pneumoniae clinical isolates at the regional teaching hospital in Yun-Lin area were 25.0 % and 24.1% in 2002 and 2003, respectively. The prevalence rate was higher than those reported from other hospitals. The aim of this study was to investigate the drug susceptibility of these isolates and examine its capability of clonal spread among them. During October 2003 to May 2004, a total of nosocomial 52 clinical isolates of ESBL-KP collected at the regional teaching hospital was enrolled for further microbiologic study. Drug susceptibilities to ampicillin, cephalothin, cefuroxime, cefotaxime, cefepime, ciprofloxacin and imipenem were determined by minimum inhibitory concentration（MIC）using E-test and its molecular epidemiology was determined by pulsed-field gel electrophoresis（PFGE）. The results showed the MIC90 value of ampicillin, cephalothin, cefuroxime, and cefotaxime was ³ 256μg/ml, that of cefepime and ciprofloxacin was ³ 32μg/ml, and that of imipenem was 0.38μg/ml. Ciprofloxacin was only effective for 17 strains（32.7%）, indicating that ciprofloxacin was not an effective choice for treating ESBL-KP. Imipenem was still the most effective antibiotic to treat ESBL-KP. The molecular epidemiology determined by PFGE showed that there were 7 strains belong to type A, 10 strains belong to type B, and the other 35 strains belong to other different minor PFGE types. Isolates classified to type A or type B needed to closely examine its capability of clonal spread among isolates in the future.
|
|---|
