Gene expression profile of hepatitis B virus-positive hepatocellular carcinomas

• Main Authors: Jhun-Ming Yeh, 葉郡銘
• Other Authors: 吳宏達 鄭如茜
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/51988712564546157195

碩士 === 中國醫藥大學 === 環境醫學研究所 === 93 === The aim of this study was to develop and validate a molecular index for the diagnosis of hepatocellular carcinoma (HCC) based on genes whose specificity and level of expression are the most discriminating for HCC. The level of expression of 30 genes was assessed by a real-time reverse transcription-polymerase chain reaction approach at Hep-G2, Huh7 and 4 HBV-positive HCCs. The most informative genes were selected as diagnostic indices. As a result, 9 out of the 30 genes were differentially expressed in HCC. Moreover, 5 out of these 9 genes were eventually selected according to their performance and univariate analysis results. A multivariate analysis was then used to obtain an expression for the diagnostic index by conditional logistic regression. Using a cut-off score of 65, sensitivity and specificity are 95% and 80%, respectively, in 20 HBV-positive HCCs. It may offer a basis for future studies on the differentiation of ambiguous cases with early-stage HCCs.