Development of Piezoelectronic Biosensor for Application in Medical Diagnosis

• Main Authors: Lin Hung-Kai, 林鴻楷
• Other Authors: Err-Cheng Chan
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/81624810792523406306

碩士 === 長庚大學 === 醫療機電工程研究所 === 93 === Quartz crystal microbalance have two characteristic is quickly test and easily operate. The goal of this study is to find the better antibody coating method and to standardize the coating protocol. We hope to by using latex beads to amplify the detection signal. We expected to detect antigen at concentration as low as 1 ng/ml. Finally, we hope to apply QCM chip in clinical test, and to supply a unexpensive and quick test. In this research, we use glutaraldehyde plus PEI method and EDC plus PEI method to coate antibody on the chip. After camparing both detection ability, we found EDC plus PEI method was better for coating because it can detect antigen at concentration of 1μg/ml. At the same time, we optimized the concentration of EDC and NHS, and the blocking protocols of glycine and BSA. Finally, we use Latex beads to enhanced the detection signals. Using latex beads, the chip can detect as low as 1ng/ml antigen. In summary, we used EDC plus PEI method is a better way to coat antibody on the chip. We also found that using latex beads, We can extend the detection limit to ng/ml. We hope this chip can be applied to the clinical test in the future.