The Research and Development of PLGA scaffold for Tissue Engineering

• Main Authors: Chun-Lien Hsueh, 薛春連
• Other Authors: 劉士榮
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/54263470799593016520

碩士 === 長庚大學 === 醫療機電工程研究所 === 93 === Tissue engineering is an interdisciplinary and multidisciplinary field. It has showed great promise in generating living alternatives for harvested tissues and organs for transplantation and reconstructive surgery. Materials and fabrication technologies are critically important for tissue engineer in design temporary, artificial extracellular materials (scaffolds), which support three-dimensional tissue formation. The report was to develop a novel solvent-free method for the manufacture of biodegradable scaffold. To manfacture a scaffold, PLGA(polylactide-polyglycolide) copolymers were pre-mixed with NaCl. The mixture was then injection compression molded to form a cylinder. Three particle sizes of NaCl (<88μm, 125-88μm, >125μm) were we used, while the PLGA to salt ratio is 1:9. Alkialine phosphatase(ALP) activity per cell were observed on cultured scaffold. The 88-125μm pore-size scaffold appears to be the most appropriate size for culturing after 7 days. The fluorometric quantification of DNA suggested that the 88-125μm size scaffold is the superior one for cell culture at 7 day. Keywords : scaffold; Tissue engineering; biodegradable;PLGA