Development of a rapid test for influenza A antigen detection

• Main Authors: ZHE-CHEN HSU, 許哲銓
• Other Authors: ERR-CHENG CHAN
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/79216224094173304045

碩士 === 長庚大學 === 生化與生醫工程研究所 === 93 === A rapid lateral-flow chromatographic membrane immunoassay test kit by using the concept of immunochromatography has been broadly applied in variety one-step immunoassay test kits,including the detection of the residual of drug and virus.There is high mortality of influenza virus in Taiwan and Hong Kong which take place by turns of season. The objective of my research is to develop a rapid lateral-flow chromatographic membrane immunoassay test kit for detection of influenza virus. We developed a rapid lateral-flow kit for influenza A virus and complete the following : colloid gold、conjugation of colloid gold and antibody、the selection of rapid lateral-flow kit and optimize of capture buffer. Sample was diluted with capture buffer before test and we can observe the result within twenty mincutes and well performance in sensitivity(0.4 ng)、specifiity and repeatable test. The activity is preserved after the drying treatment in the condition for 24 hour、 75˚C.