| Summary: | 碩士 === 長庚大學 === 天然藥物研究所 === 93 === Lysophosphatidic acid (LPA) is one of bioactive phospholipids which play a regulatory role in a wide range of physiological functions such as proliferation. However, the mechanisms underlying LPA-induced cell proliferation in tracheal smooth muscle cells (TSMC) remain unclear. We report that the mitogen-activated protein kinases (MAPKs) and Akt pathways participated in cell proliferation induced by LPA in TSMC. LPA increased cell proliferation, which was attenuated by inhibitors of MEK1/2 (U0126), EGFR (AG1478), PDGFR (AG1296), PI3K (wortmannin), and Ca2+ chelator (BAPTA). Accordingly, LPA induced phosphorylation of p42/p44 MAPK and Akt which was attenuated by U0126, LY294002, or wortmannin, respectively. These results suggest that in TSMC, activation of p42/p44 MAPK and Akt pathways is essential for LPA-induced cell proliferation. Understanding the mecahnisms involved in LPA-induced cell proliferation on TSMC may provide potential therapeutic values in the treatment of airway diseases.
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