Molecular characterization of cyclin B and CDC2-relate kinase in Trichomonas vaginalis

• Main Authors: HUANG-CHUNG SHENG, 黃忠勝
• Other Authors: 鄧致剛
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/11881048359255741494

碩士 === 長庚大學 === 基礎醫學研究所 === 93 === Cell cycle control by CDC2-related kinase (CRKs) and cyclins are essential to the regulation of cell proliferation and developmental process in many organisms. Here we have cloned the CRK2 and CYC genes from T. vaginalis, which encodes a 36.7kDa, 38.3kDa proteins respectively. Although both amino acids sequences have low level of sequence similarity to CDC2 and mitotic B-type cyclin from a variety of organisms, but that the presence of several specific conserved motif/domains and the 3-D structure revealed that the Trichomonas proteins are highly homologous with human and parasite. The TvCYC and TvCRK2 are multiple copy genes, and expressed in T. vaginalis In order to demonstrat whether CRK2 and CYC protein possess most of functional homolog properties of CDC-relate kinase and cyclin B, we generated fusion proteins His-TvCYC and His-TvCRK2. Western blots with anti-PSTAIRE and anti-His antibody is shown a single band of approximately 43.5kDa for His-TvCRK2 fusion protein. Polyclonal antibody against His-TvCYC was prepared from rat and was able to detect endogenous CYC. These data indicated that T. vaginalis have cyclin and CDC2-related kinase homologues. A complex mechanism may exist in order to regulate the kinases involved in the cell cycle and the differentiation processes of the parasite.