| Summary: | 碩士 === 長庚大學 === 化工與材料工程研究所 === 93 === Biodegradable nanofiber membranes from poly-L-lactic acid (PLLA) were prepared by electrospinning and used as a scaffold for cartilage tissue engineering. Operating parameters during the electrospinning process were studied in details in terms of fiber diameter and pore size of the scaffolds. The PLLA nanofiber membanes were characterized by SEM, FESEM, BET, SPM, and TEM. In order to improve cell attachment and growth, PLLA membranes were subjected to DC-pulsed oxygen plasma treatment, followed by acrylic acid grafting and collagen coating by covalent binding of collagen to carboxylic moieties of the polyacrylic acid. The bioactive scaffolds could be used for culture of chondrocyte with improved cell viability, proliferation, and differentiation.
Primary chondrocyte cells seeded onto the PLLA nanofiber membranes could maintain their viability by MTT assay. Cell functionabilty was confirmed by secretion of GAG and collagen during the cultivation period up to 1 month. Scanning electron microscopy (SEM) were used to confirm the morphology and structure of chondrocytes.
In conclusion, the collagen-grafted PLLA nanofiber will be a useful scaffold for studying cell-material interactions with its nanofibrous nature and microporous ultrastructure and has a great potential to be used as a scaffold for tissue engineering applications.
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