| Summary: | 碩士 === 國立中正大學 === 物理所 === 93 === We construct a hybrid system, which combines differential confocal microscopy and optical tweezers with lateral and axis resolution. The Brownian motion of a bead was tracked precisely to verify the function of the system.
This system can do the real time and 3-dimensional measurement with the diameter of sample bigger than the wavelength of light source and without the complex image analysis. The factors of the sample that is fluorescent or with high scattering coefficient are required less. While working with feed-back control system, the whole system can track the area wider than trapping beam.
When the 3 μm beads are trapped by trapping beam, the scale of Brownian motion in axial direction is about one order higher than in lateral one. The lateral Brownian motion ranges the zero point about 15 nm with 11 mW trapping power and about 54 nm with 1.4 mW. I change the power of trapping force, but the range of Brownian motion dose not change very much. This system can prove that the trapping force in lateral direction is great than axial, and also prove that the decrease power of trap force can increases the range of Brownian motion of the bead.
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