Patterned polyelectrolyte multilayer surface for the manipulation of cell alignment

• Main Authors: Chien-An Chen, 陳建安
• Other Authors: Yi-Ming Sun
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/73679351383162438356

碩士 === 元智大學 === 化學工程學系 === 92 === In this study, soft lithography technique was applied to create micropatterns on substrate surface for the manipulation of cell adhesion and growth. Micropatterns were first fabricated on stainless steel plates or silicon wafers via photolithographic technique. By using these topo- logically-patterned substrates as masters, complementary poly(dimethyl- siloxane) (PDMS) replicas were prepared. The PDMS replicas were used as pattern transfer elements (stamps) in microcontact printing (mCP), and their spatial resolution could reach 10 mm. Combining layer-by-layer (LBL) and mCP techniques, oppositely charged polyelectrolytes, poly(allylamine hydrochloride) (PAH) and poly(acrylic acid) (PAA), were alternatively deposited onto cover glasses for 6 to 10 layers, and chemically differentiated patterns of PAH/PAA were on the topmost layer. Hydrophilic polyethylene glycol (PEG) moiety was grafted onto the PAH region through PEGylation with an amine-reactive coupling reaction. Finally, the topmost layer became PEG/PAA patterned surface. C3A cells selectively adhered and grew on the PAA region of the patterned surface in serum-containing medium and the spatial resolution could reach 50μm.