| Summary: | 碩士 === 國立陽明大學 === 醫學生物技術研究所 === 92 === The receptor for advanced glycation end products（RAGE）is a member of the immunoglobulin super family of cell surface molecules, and the biology of RAGE driven by its ligands is associated with inflammatory responses. And it has been defined as a target of drug design to reduce the inflammatory disorders of diabetes, recently. Folliculo-stellate cell（FS cell）, the non-hormone secreting cell of anterior pituitary gland, could produce many cytokines or growth factors which could influence the hormone production and modulate the immunoendocrine connections. Since the biological function of RAGE of FS cell had not been discussed, we investigate if RAGE participates in the inflammatory responses of FS cell. By using a FS-like cell line, TtT/GF, as a model, we first found that TtT/GF cell expressed RAGE, which was up-regulated by LPS at both mRNA and protein level. Horever, a ligand of RAGE, S100B, which normally expressed on FS cells, was not regulated by LPS at both mRNA and protein level. We also found the distribution of RAGE on the membrane was clustered after LPS stimulation. LPS could stimulate the actin bundling and the assembling of free form α-tubulin in the cytoplasm toward to the nucleus. Protein expression of α-tubulin was also up-regulated by LPS. We suggest that the expression of RAGE up-regulated by LPS may couple to inflammatory responses of FS cell. The distribution of RAGE altered by LPS may drive by its ligands except S100B, and may involve in the mobility of FS cell with cytoskeletons. We have build the siRNA construct of RAGE, and it may be useful to investigate the biological significant of these changes in the future.
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