Expression profile and methylation pattern of imprinted genes in liver regeneration

• Main Authors: Jia-Liang Jiang, 姜佳良
• Other Authors: Ann-Ping Tsou
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/59041921112671804809

碩士 === 國立陽明大學 === 醫學生物技術研究所 === 92 === Imprinting is an important epigenetic regulation. Cells can distinguish parental origin of different alleles and express the correct one. As a recognition marker, most of the imprinted genes have differentially methlyated region (DMR) on the two alleles. Imprinted genes play important roles in embryonal development and behavior. We previously reported Peg10, an imprinted gene that was expressed at 48 hours to 96 hours after partial hepatectomy (PHx) but a systematic investigation of imprinted genes in liver regeneration has been lacking.We combined the information from several public databases of imprinted genes with a microarray dataset of liver regeneration to identify expression of many candidate imprinted genes. These genes have potential to participate in regulation of cell growth and apoptosis based on their known biological functions. In an attempt to correlate the methylation patterns at DMR with gene expression, four genes, Dlk1,Grb10, Mest and H19 were studied in this work. We found no detectable variation of methylation pattern at DMR of Dlk1, Grb10 and Mest while significant reduction of DMR methylation was found in H19 at PHx 36h and 96h. Since the expression level of H19 did not correlate linearly with the altered pattern of methylation, we speculate that other mechanisms such as regulation by cirtical transcription factors may play important role in controlling gene expression of H19. This is the first time that such a global strategy was applied to explore the methylation status of potentially imprinted genes during liver regeneration.