| Summary: | 碩士 === 國立陽明大學 === 解剖暨細胞生物學研究所 === 92 === Spinal cord transection results in permanent deficits involving complete paralysis and sensory loss below the level of lesion. Olfactory ensheathing cells (OECs) are regarded as the best therapeutic agent for use in the repair of spinal cord injury. We successfully established OEC with over 95% cells expressing GFAP, S100 and p75NGFR.
Neurotrophin-3 (NT-3), a putative neurotrophic factor in the rubro- and cortico-spinal tracts has been shown to effectively promote regeneration of damaged axons into the spinal cord. However, its expression is low in OEC. The present work therefore examines the effects of OEC transplant combined with fibrin glue mixed with NT3 on functional recovery after T8-transection.
OEC, prelabelled with 5-bromo-2-deoxyuridine or green fluorescence protein, were mixed with fibrin glue with/without NT-3 and transplanted into the transected spinal cord. Behavior tests (BBB and CBS) and were conducted every other week. By post operation , significant functional recovery in hind limb usage occurred in OECs or OECs + NT3 transplanted rats groups compared with control groups, transplanted with HBSS alone(p<0.05 ANOVA). However, none of the animals demonstrated coordinated fore and hind limb movement or the ability to bear weight on the hind limbs.
The immunhistochemistry showed that, the T7 level of OECs + NT3 groups presented more neurofilament and serotonin marker than control groups. At the transected site, neurofilament and serotonin marker can be found in the OECs + NT3 groups but not in the control groups.
In conclusion, our results demonstrated that (1) The primary cultured cells of OECs from olfactory bulb can present the GFAP, S100 and p75NGFR. (2) Fibrin glue enwrapped OECs with/without NT-3 transplanted rats showed significantly higher functional recovery in hind limb. (3) Fibrin glue enwrapped OECs with/without NT-3 transplanted rats facilitated the newly regenerated axon to pass the transected site.
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