Anti-proliferation and radio-sensitizing effect of caffeic acid phenethyl ester on human Daoy medulloblastoma cell line

• Main Authors: Wen-Ser Tseng, 曾文瑟
• Other Authors: Jen-Hwey Chiu
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/01080141749648728526

碩士 === 國立陽明大學 === 傳統醫藥學研究所 === 92 === Abstract Medulloblastoma (MB) is the most common malignant cere- bellum tumor in children. Despite surgical eradication, chemotherapy and radiotherapy, the prognosis of MB is poor. To improve current treatment prognosis in MB patients, the discovery of novel therapeutic agents become a urgent issue. Caffeic acid phenethyl ester (CAPE), an active component of propolis, is known to have antioxidant, anti-inflammatory, antiviral, reperfusion injury prevent, anticancer and radio-sensitizing effects. This study is aimed to investigate the effect of anti-proliferation and radio-sensitization of CAPE on MB Doay cell line. Our data show that CAPE inhibited the growth of Daoy cells in a time- and concentration-dependent manner by MTT assay and Trypan blue exclusion assay. Flow cytometry assay revealed that treatment of Daoy cell with CAPE significantly decreased the percentage of G2/M fraction, and increased the S phase fraction. The results suggest that CAPE delay cell cycling from S to G2/M phase. Furthermore, Western blot analysis could demonstrate a down-regulated cyclin B1 protein expression in CAPE-treated Daoy cells. Furthermore pretreatment with 0.1~10μM CAPE markedly decreased the survival of irradiated Daoy cells. The SERs at 37% survival were 1.19、1.32、1.50、2.04 in Daoy cells for 0.1, 0.3, 1, 3μM CAPE, respectively. It is concluded that CAPE is an effective proliferation inhibitor and radiosensitizer for Daoy cells. It has potential as an adjunct to the clinical adjunctive therapy for human medulloblastoma.