Probing interactions between the cytoskeleton and the endocytic vesicles using Total Internal Reflection Fluorescence Microscopy
碩士 === 國立陽明大學 === 生醫光電工程研究所 === 92 === Molecular interactions taken place near or on the cellular plasma membrane play an important role in maintaining cellular physiology. Moreover, molecular interactions on the plasma membrane are often expected to have different properties from those occurring in...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2004
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| Online Access: | http://ndltd.ncl.edu.tw/handle/72090950181188827554 |
| Summary: | 碩士 === 國立陽明大學 === 生醫光電工程研究所 === 92 === Molecular interactions taken place near or on the cellular plasma membrane play an important role in maintaining cellular physiology. Moreover, molecular interactions on the plasma membrane are often expected to have different properties from those occurring in bulk solutions. Fortunately, total internal reflection fluorescence microscopy provides a good way to probe these important biological processes near plasma membrane with a much higher image quality than other optical microscopy. Using TIRFM, we can visualize the interactions between the cytoskeleton and the endocytic vesicles near the cellular growth substrate simultaneously at a higher signal to noise ratio.
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