Fiber Optic Biosensor For Real Time Monitoring Protein Binding Kinetics

• Main Authors: Chih-Jen Yu, 游智仁
• Other Authors: Chien Chou
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/45312132106992113590

碩士 === 國立陽明大學 === 生醫光電工程研究所 === 92 === In cell biological research, protein control mediates many of biological activities of cells. Most of proteins function in association with other protein to gain an cellular function. Therefore protein-protein interaction becomes essential to understand biological activities that have been studied by determination of kinetic constants. The fiber optic biosensor is one of the easiest way to setup to study the kinetics of protein-protein interaction in real time where the optical fiber is disposable in the measurement. Then fiber optic biosensor is a novel equipment for kinetic constants measurement. C-reactive protein (CRP), an acute phase protein of inflammation is related to the risk factor for cardiovascular diseases. In recently study, a different expression of CRP, the modified CRP (mCRP), shows more dependence on cardiovascular diseases significantly than CRP. In this study, a fiber optic biosensor was setup in order to determine mCRP/anti-CRP binding kinetics to investigate the condition between mCRP and the risk factor of cardiovascular diseases quantitatively. The experimental result will be useful for the purpose of earlier diagnosis of the cardiovascular diseases.