Optimization of Submerged Cultivation of Monascus anka via Sequential Pseudo Uniform Design Method

• Main Authors: Jinn-Tsair Lee, 李進財
• Other Authors: Jyh-Shyong Chang
• Format: Others
• Language: en_US
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/98078046212648591348

碩士 === 大同大學 === 化學工程學系(所) === 92 === The experiments of submerged cultivation of Monascus anka were carried out according to the SPUD design. The control factors were the cultivation temperature and the dissolved oxygen. The measurements of glucoamylase activity and concentration of red pigment from the experiments were used to identify a second-order response model. Eleven experiments located by the initial UD method followed by the SPUD method were used to build the second-order response model with 2.71% average modeling error for the glucoamylase activity but 12.44% average modeling error for the concentration of red pigment. Two additional experiments could not improve the quality of the second-order response model describing the concentration of red pigment. Considering the time resource for carrying out the experiments, the sub-optimal operating condition was determined based on available experimental data to achieve the maximal overall desirability objective function of glucoamylase activity and concentration of red pigment of the end product. The experimental results shown in this work demonstrated the applicability of the SPUD method to locate limited experiments for guiding the experimenter to reach a sub-optimal or optimal operating condition of an unknown process.