The study of the nucleic acid chaperone activity of the N-terminal domain of hepatitis delta antigen

• Main Authors: You-Ju Chen, 陳幼襦
• Other Authors: Huey-Nan Wu Ph.D.
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/20819428168883849377

碩士 === 臺北醫學大學 === 細胞及分子生物研究所 === 92 === The N-terminal domain, which contains amino acids # 1-88, of hepatitis D antigen possesses a nucleic acid chaperon activity. In this study I investigated the molecular mechanism of the nucleic acid chaperon activity of NdAg. I utilized the Tm analysis to examine the thermal stability of chemically synthesized DNA duplexes, and then choosed specific DNA oligomers to analyze the nucleic acid chaperon activity of NdAg, namely the selective strand annealing activity and the selective strand exchange activity. I found that NdAg facilitates the formation of more stable duplex among competing sequences of high as well as low Tm value ranges, and NdAg effects in different DNA oligomer concentrations. Besides, NdAg also promotes strand exchange for the formation of more stable DNA duplex, and the strand displacement process does not have a specific polarity.