| Summary: | 碩士 === 臺北醫學大學 === 細胞及分子生物研究所 === 92 === Acute leukemia is characterized by accumulation of malignant blasts in bone marrow. Leukemic cells have abnormal differentiation. Acute leukemia can be grouped into myeloid and lymphoid types. The exact cause of leukemia is still not known. Acute myeloid leukemia is the most common leukemia type in adult and is categorized into eight subtypes (M0-M7) according to the French-American-British (FAB) classification. AML studies rely heavily on the availability of various cell lines. Most of the AML cell lines are derived from Cauacsians. No AML cell line is derived from Taiwanese. In this study, we successful established a leukemia cell from the bone marrow of a AML-M2 patient. This cell line could growth in 10% IMDM medium and maintain the meyloblastic morphology. The cells expressed high CD33,CD14 surface marker and low CD3,CD19 marker. Treatment with retinoic acid or 1α,25- dihydroxy vitamin D3 induced cells to differentiate into neutrophils or monocytes, respectively. Karyotyping study showed multiple abnormalities. In the NOD/SCID xenogeneic animal study, we found that this cell line could generate granulocytic sarcoma in vivo. We believe that this is first AML cell line ever established from Taiwanese. We hope that this cell line will be useful for AML studt in the future.
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