Development of Lipid Panel Biosensors

• Main Authors: Wei Chung Shih, 施偉仲
• Other Authors: Meng Shan Lin
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/58706271544596901094

博士 === 淡江大學 === 化學學系 === 92 === The goal of this dissertation is to develop a portable electrochemical lipid biosensors for cholesterol, cholesteryl ester, triglyceride, and high density lipoprotein cholesterol respectively. These biosensors can be applied to evaluate the lipid panel and the risky factor for cardiovascular disease in clinical medicine in the future. The integrated three electrode system was screen printed on the top of PP sheet and the electrochemical signal was measured by a home-made portable potentiostat. To improve the selectivity and sensitivity, the sensing strip included the catalyst layer and the enzyme reaction layer to reduce the applied potential and recognize the analyte, the operation conditions were carefully studied. The sensor strips targeting the free cholesterol, total cholesterol (TC), triglyceride(TG) and high density lipoprotein cholesterol (HDL-C) were based on the measurement hydrogen peroxide concentration from above three biosensors and the value of LDL-C can be calculated with reported Friedwald equation, (LDL-C) = (TC)-(HDL-C)-(TG/5), and risky factor equal to (TC)/ (HDL-C) also can be evaluated. The result shows that the detection ranges of biosensors are up to 300 mg/dL for free cholesterol, 500 mg/dL for cholesteryl ester, 750 mg/dL for triglyceride and 110 mg/dL for high density lipoprotein cholesterol. The detection ranges of these electrochemical biosensors are fit for the most clinical requirements. The detection limits according to priority are 17.6, 19.4, 28.2, and 23.2 mg/dL and the measurement time are 25, 20, 65, and 35 sec. These characteristics meet the requirement of clinical needs for lipid assessment.