Studies on the production of biosurfactant by Acinetobacter calcoaceticus RAG-1

• Main Authors: Yi-Fan Lin, 林逸凡
• Other Authors: Wen-Jang Chen
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/52641104476434934557

碩士 === 國立臺北科技大學 === 化學工程系碩士班 === 92 === Biosurfactants have several advantages over the chemical surfactants, such as lower toxicity; higher biodegradability and better environmental compatibility. Therefore, its effluent water have low pollution for environment, and it has great potential for application. In this work, the application of experimental design for the biosurfactant production by Acinetobacter calcoaceticus RAG-1 from ethanol as sole carbon source were studied in shaking flasks. Then the effect of the rates of agitation in jar fermentor on the biosurfactant production, were studied in relation to the physical and chemical properties, and biodegradability of the biosurfactant. Finally, the microbial growth kinetics and biosurfactant production kinetics were modeled by using Monod equation and the Luedeking and Piret equation, respectively. The experimental results showed that in the shake flasks the optimal medium compositions were 9.16 g/L ethanol, 8.2 g/L KH2PO4, 23.32 g/L K2HPO4, 5.77 g/L (NH4)2SO4 and 0.354 g/L MgSO4．7H2O and the biosurfactant production of 73.312mg/L was obtained after 80 hours of cultivation. The biosurafctant yield were not changed by the rates of agitation in fermenter over 600-800 rpm. According to the Luedeking and Piret model, the bacterial production of biosurfactant was found to be non-growth associated. The biosurfactant had good biodegradation, approximately 60% degradation by activated sludge.