| Summary: | 碩士 === 東海大學 === 環境科學系 === 92 === The thermophilic community structure was investigated in this study at Lulu hot spring and Shihsankeng hot spring. For this investigation, it based on the analysis of PCR amplification containing the 16S ribosomal RNA gene.
Two types of libraries were generated in this study: DGGE libraries analysis of the variable region (V3-V5), which were further analysed by excising, reamplification, sequencing, and random shotgun sequence libraries. Phylogenetic and sequence similarity analyses of the resultant in DGGE libraries revealed the presence of 24 operational taxonomic units (OTUs) or phylotypes, which were defined as having more than 98% of sequence similarity. 80 clones (40 clones from each sample site) sequences in shotgun libraries revealed 28 OTUs.
The similarity, diversity, and evenness in these libraries were estimated using a variety of diversity indices. Shinsankeng exhibited more diverse and variegated bacterial populations. Phylogenetic analysis confirmed the diversity of thermophilic divisions. The common group in two communities appeared to include Clostridia, Chroococcales, β-proteobacteria, Spirochaetes and CFB (Cytophaga- Flavobacterium- Bacteroides ).
Cultivation was used to isolate pure cultures under 75℃ from 2 sample sites. Among 100 pure strains, 22 phylotypes were distinguished by RFLP (Restriction Fragment Length Polymorphism).
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