Reductive Ability of Porcine Liver and its Enzymatic Hydrolysate Powder on Non-heme Iron

• Main Authors: Wen-Chuan Chu, 朱文全
• Other Authors: Dr.Chi-Fa Chow
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/29472855289866971340

碩士 === 東海大學 === 畜產學系 === 92 === 英 文 摘 要 Reductive Ability of Porcine Liver and its Digestive Peptide Powder on Non-heme Iron. Wen-Chuan Chu Abstract The purpose of this study was to manufacture high protein powder from porcine liver by enzyme digesting to increase its solubility in water and the reductive ability of ferric iron toim prove the absorption of non-heme iron in human body. The fresh porcine liver samples were bought from traditional market and stored at 4℃. The samples were divided into whole fresh liver(FL), water-soluble, salt-soluble and insoluble fractions followed without or with enzyme digestion, then those frozen dried power were analyzed the general composition, the weight of percentage, the relative solubility in different pH-value and the reductive ability of ferric iron. In result, FL was the highest in moisture(P＜0.05). Dry whole fresh liver(DWFL) and digested dry whole fresh liver(DDWFL) were the highest in ash(P＜0.05). DWFL was the highest in crude fat(P＜0.05). Water-soluble fraction(WPF) and digested water-soluble fraction(DWPF) were the highest in crude protein(P＜0.05). DWFL and DDWFL have the highest percentage of dry weight, but DWPF and digested salt- soluble fraction(DDSFL) were slightly increased and digested insoluble fraction(DIFL) decreased slightly. When compared the relative solubility of each group with WPF or DWPF at pH3.0, all fractions were below 20% from pH4.0-5.0. The relative solubility of WPF, SPF, DWPF and DSPF were equal around 75% in pH6.0-10.0. Each fraction of porcine liver powder treated with enzyme could raise the relative solubility about 40-50% than that without enzyme digested . The relative solubility of DWPF at pH4.0-5.0 were around 60% and other pH were over 120%. The fraction without enzyme treatment, WPF had the highest ferric iron reductive ability on the concentration at 0.05%, and DWPF needed to reach 0.1%. The ferrous iron reductive ability between WPF at 0.05% and DWPF at 0.1% were no significant difference(P＞0.05).