nvestigating the Changes in Chemical Compositions and Toxicity of Aconiti carmichaeli Radix Praeparata During Processing and Their Biological Implications

• Main Authors: Mei -Chou, 賴美州
• Other Authors: none
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/28576949191583186434

碩士 === 大仁科技大學 === 環境管理研究所 === 92 === Abstract The active ingredients of the Chinese herb are too complex to evaluate. One prescription used in the Chinese traditional medicine might use to cure different disorders. The ingredients extract from an herb will be different with different preparation. The therapeutic effect will be potentate with proper preparation; however, the toxicity might be obtained if the preparation is not well. Therefore, the quality control in the preparation of Chinese herbs is very important. Preparation is important in Chinese traditional medicine, almost clinical use are about preparation. In the present study, the botanical origin of the Fuzei (Aconitum carmichaeli Debx.) obtained from Chengdu, the capital of Sichuan Province, was investigated by physiognomy, anatomy method, Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC). Then, crude Fuzei was prepared to Salt-Fuzei, Hei-Swong-Peng and Pai-Swong-Peng to compare the toxicity and pharmacology activity between these preparations. The content of aconitine in Fuzei without preparation was about 0.05 mg/g obtained from quantitative analysis by HPLC. However, the contents of aconitine in Salt-Fuzei, Hei-Swong-Peng and Pai-Swong-Peng were 0.006 mg/g、0.002 mg/g and 0.002 mg/g , respectively. The content of lappaconitine in Fuzei without preparation was about 0.119 mg/g obtained from quantitative analysis by HPLC. However, the contents of lappaconitine in Salt-Fuzei, Hei-Swong-Peng and Pai-Swong-Peng were 0.0003 mg/g、0.005 mg/g and 0.004 mg/g , respectively. The median lethal dose（LD50）in Fuzei without preparation was 1.4 g/kg. In contrast, the LD50 was increased to 9.9 g/kg, 10.6g/kg and 20.2 g/kg in Salt-Fuzei, Hei-Swong-Peng and Pai-Swong-Peng, respectively. Fuzei possess the ability to increase the threshold of pain that is centrally mediated by opioid receptors. Among the processed products, the analgesic effect of Yan-Fuzei was the most potent, which was nearly that obtained from crude Fuzei. Oral administration of streptozotocin-induced diabetic rats (STZ-diabetic rats) with Fuzei at the dosage of 50 mg/kg for 2 h, the plasma glucose lowering activity of Fuzei without preparation, Salt-Fuzei and Hei-Swong-Peng were 17.8 ± 2.7 %, 16.3 ± 2.4 % and 17.6 ± 3.1 %, respectively. However, the plasma glucose lowering activity of Pai-Swong-Peng in STZ-diabetic rats was only 0.8 ± 1.8 %. In fact, the concentrations of aconitine were significantly less in processed Fuzei than that in crude Fuzei. The biological effect of Yan-Fuzei, the salt baking product, was most potent among the processed products. The present study will be helpful in clinic to apply Fuzei safety. Key words : Fuzei ，preparation，Salt-Fuzei ，Hei-Swong-Peng，Pai-Swong-Peng