Effects of dietary vitamin E and exercise training on oxidative and antioxidative statuses in studied rats

• Main Authors: Hsin-Ju Lee, 李欣儒
• Other Authors: Tim K. Tso
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/08787792767425881914

碩士 === 實踐大學 === 食品營養研究所 === 92 === It has been recognized that free radical production is increased after moderate to high levels of exercise. Elevated free radical has been linked to premature fatigue and development of various diseases. Antioxidant defense system in the body can be induced by regular training to counteract the elevated free radical production during exercise. However, most studies so far have focused on the changes after a single bout or a period of training. The changes of oxidative and antioxidative statuses, which are composed of different muscle types throughout a long term exercise programs, have not been investigated longitudinally. The objective of this study was to assess the effects of dietary vitaminE and exercise training on oxidative and antioxidative statuses in rats, and comparison of different muscle types affected by oxidative and antioxidative statuses. There were thirty adult male Sprague-Dawley rats in this study. The training program was gradually increased to keep the workload at about 75% of maximal oxygen consumption for 90 min for 5 days per week for 8 weeks. Dietary vitamin E diet was prepared by mixing α-tocopherol acetate with grounded non-vitamin E diet to the level of 75 U/kg diet. The rats were randomly divided into four groups including dietary vitamin E combined with training (YEYT group, n=8), dietary vitamin E without training (YENT group, n=8), non-dietary vitamin E combined with training (NEYT group, n=4) and non-dietary vitamin E without training (NENT groups, n=10). According to different muscle types, rats were divided into four groups, which were white quadriceps (WQ group), red quadriceps (RQ group), white gastrocnemius (WG group), red gastrocnemius (RG group). Oxidative statuses including thiobarbituric acid reacting substances (TBARs) and oxidative protein level and antioxidative statuses including superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GRd) and catalase (CAT) were determined in each group. Results showed that TBARs of the NEYT group was most severe, and oxidative protein level of the non-dietary vitamin E groups (NEYT and NENT groups) was slightly higher than other dietary vitamin E groups. In NEYT group, SOD activity was significantly lowest, but GPx activity was significantly highest. Muscle content of vitamin E was higher in dietary vitamin E groups. Effects of different muscle types showed that TBARs was only associated in fiber type. RQ and RG groups were significantly higher than WQ and WG groups. The muscle type did not affect oxidative protein levels. Antioxidant enzyme SOD activity was affected by interaction of muscle parts and fiber types. RQ group was significantly higher than WQ, WG and RG groups in SOD activity. Muscle parts and fiber types affected GPx activity. RG group was significantly higher than WQ, RQ and WG groups in GPx activity. GRd and CAT activities in gastrocnemius groups (WG and RG) were significantly higher than quadriceps groups, and red fiber types (RQ and RG groups) were significantly higher than white fiber types. Vitamin E content in gastrocnemius muscles was significantly higher. In summary, NEYT group or red fiber types groups had higher oxidative stress with lower SOD activity and higher GPx, GRd and CAT activities than others.