Preparation and Hemocompatibilty of Water-Soluble Chitosan Co-ionic Complex Beads

• Main Authors: Lin, Yie-Hsean, 林業祥
• Other Authors: Yang, Ming-Chien
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/10076634442976877618

碩士 === 國立臺灣科技大學 === 高分子工程系 === 92 === Co-ionic complex beads were prepared by precipitating chitosan using tripolyphosphate (TPP) and dextran sulfate (DS). Water-soluble chitosan (WSC) of two molecular masses (99 and 181 kD) and common chitosan (CS) were employed in this study. These beads were characterized using elemental analysis (EA), optical microscopy, and scanning electron microscopy (SEM). The swelling kinetics and the distribution coefficients of three compounds were also measured. The hemocompatibility was evaluated based on the protein adsorption, platelet adsorption, activated partial thrombin time (APTT), and prothrombin time (PT). The results showed that beads made from WSC exhibited higher swelling ratio (SR) than from CS. The SR increased with the DS content and depended on the pH. Beads made from WSC also exhibited lower cytotoxicity than beads from CS. The biodegrading rate by lysozyme of WSC beads was faster than that of CS beads. In addition, WSC/TPP/DS beads exhibited less protein and platelet adsorption, and longer APTT and PT. These results indicated that the addition of DS could endow WSC and CS beads with anti-coagulating ability.