Combination of Pulsed-Field Gel Electrophoresis andSingle-Enzyme Amplified Fragment Length Polymorphismfor Differentiation of Multiresistant Salmonella entericaserovar Choleraesuis

• Main Authors: Chen-Yi Chien, 簡禛誼
• Other Authors: Chao-Fu CHUNG
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/40030168918938750261

碩士 === 國立臺灣大學 === 獸醫學研究所 === 92 === Salmonella enterica serovar Choleraesuis (SC) is an important bacterial pathogen, which is usually causes septicaemia, pneumonia and diarrhea in nursery or grower pigs and systemic infection in humans bacteremia and metastatic focal infections. Recently, it has been reported showed that most of the swine and human Salmonella isolates had mutiresistant. To trace the clonal relationship between swine and human isolates. For their antimicrobiol agents、pulsed-fied gel electrophoresis (PFGE) and Single-Enzyme Amplified Fragment Length Polymorphism (SAFLP) were used to differentiate the multiresistant pattern of the 97 isolates with thirty-five different antibiograms. However, were five different antimicrograms, which were ACepCECpLGNaSSxtT, ACECpLGNaSSxtT, ACepCECpLNaSSxtT, ACECpLNaS- SxtT, ACGNaSSxtT. Their are isolates with the same antibiogram but are different plasmid and PFGE profiles. Fifteen of the swine isolates and fourteen of the human isolates shared five different antimicrograms. Sixty-nine plasmid profile、fifty-one PFGE profiles and sixty SAFLP profiles were detected from the 97 SC isolates. Most of the animals and human strains showed different PFGE pattern.We also found that 15 swine and 8 human isolates shared the same SAFLP pattern. In conclusion, all of isolates have different plasmid and PFGE profiles. 15 swine and 8 human shared a common SAFLP pattern. All 97 isolates had different in combination PFGE and SAFLP genotyping. The application of antibiogram, plasmid profile, PFGE and SAFLP in dicated that not all human isolates came from swine.