Transcript screening for very late genes of white spot syndrome virus(WSSV)

• Main Authors: Der-Ming Yi, 易德明
• Other Authors: Chu-Fang Lo
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/77461747913562540108

碩士 === 國立臺灣大學 === 動物學研究研究所 === 92 === Microarray of time-course white spot syndrome virus (WSSV)-infected Penaeus monodon presents a transcription profile. The result reveals 13 open reading frames(orfs) that transcribed at approximately 36 hours post infection. Temporal analysis of 13 orfs by RT-PCR confirms the result of WSSV microarray and reveals that 8 orfs may be the very late gene candidates of WSSV. Motif and domain prediction results of WSSV very late gene candidates significantly discovered three types of regions that may contribute to the phenomena of WSSV infection very late stage. WSSV very late gene may function as anti-apoptosis genes or host immune evasions, which were advantageous in virion replication. Discovery of WSSV very late gene candidates provide a novel field of WSSV genomic research. In addition, functional analysis of WSSV very late gene candidates gives more implications about biological interaction between WSSV and its hosts. WSSV very late gene candidates may be crucial factors for providing solutions for the epidemic of WSSV.