Evaluation of avidin-biotin binding system in chondrocyte adhesion and proliferation

• Main Authors: Min-Cheng Wang, 王閔正
• Other Authors: 蔡偉博
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/77613683247733597044

碩士 === 國立臺灣大學 === 化學工程學研究所 === 92 === Cell adhesion to synthetic biomaterials is a prerequisite for anchorage cell culture and tissue engineering. The current study investigated the utilization of the avidin-biotin binding system in enhancing chondrocyte adhesion to tissue culture polystyrene (TCPS) and biodegradable polymer surface. Porcine chondrocytes were biotinylated by using 3-Sulfo-NHS-Biotin, and avidin was immobilized onto material surface by adsorption. In this study, we revealed that avidin-biotin system can enhance initial chondrocytes adhesion and spread out in a short time. The progeny of biotinylated chondrocytes still maintained the ability of expressing cartilage extracellular matrix proteins, such as type II collagen and aggrecan. Otherwise, we also used avidin-biotin system with fibronectin on PLGA surface. Contrast with using combine system and single system, combine system can enhance cell adhesion than avidin-biotin system and fibronectin system. After seven days culture, the progeny of these cell still maintain the ability of expressing cartilage extracellular matrix proteins. These results indicate that the avidin-biotin binding system could therefore help in the area of cell culture and tissue engineering.