Study of Yeast Cin5p Interaction with Hms1p

• Main Authors: Andy Yen-Chin Wang, 王彥欽
• Other Authors: Shu-Chun Teng
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/30255603633471421285

碩士 === 國立臺灣大學 === 微生物學研究所 === 92 === To study the characteristics of Saccharomyces cerevisiae Hms1 transcribing Hsl7p, we used Hms1 as the bait for a yeast two-hybrid system screen. Cin5 was discovered to interact with Hms1. Further attempts of research included Cin5 TAP-tag purification as well as in vivo coprecipitation of Cin5-HA and Hms1-myc. Due to expression amount the tagged Cin5p couldn’t be successfully detected. An in vitro coprecipitation experiment was also carried out to confirm the interaction between Cin5 and Hms1 using an E. coli expression system with His and GST tags. Western blotting results do not support the interaction between Cin5 and Hms1.