Design of a Multiple-Throughput Device for Electrophysiological Screening
碩士 === 國立臺灣大學 === 應用力學研究所 === 92 === The patch clamp technique, in the standard electrophysiological assays, is a well-developed and powerful tool for the study of ion channels and transporters. Despite claims that the patch clamp technique has superior characteristics of high information content, t...
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| Format: | Others |
| Language: | zh-TW |
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2004
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| Online Access: | http://ndltd.ncl.edu.tw/handle/87555968314927350708 |
| Summary: | 碩士 === 國立臺灣大學 === 應用力學研究所 === 92 === The patch clamp technique, in the standard electrophysiological assays, is a well-developed and powerful tool for the study of ion channels and transporters. Despite claims that the patch clamp technique has superior characteristics of high information content, the technique is suffered from by the laborious and manual manipulations of glass pipette onto the cell by highly skilled technicians with consequential low throughput.
This thesis presents an integrated multiple-cell patch-clamp device for ion channel study. The recording chamber is an acrylic multi-layer structure and the cell-attached portion being polymeric polydimethylsiloxane (PDMS). The elastomeric PDMS is an attractive material to mimic the tip of a glass micropipette that readily forms gigaohm seals to a cell membrane after surface treatment. The recording system not only avoids the inherent shortcomings found in the conventional patch clamping but also provides a multiple-throughput apertures for electrophysiological recording to raise the throughput of data points.
I demonstrated that the PDMS recording chip provided patch clamp recording of Xenopus oocytes expressing inactivation-removed Shaker potassium channels at different apertures of the recording chip asynchronously. The results indicate the potential for high-throughput electrophysiological screening with an integration of our recording system.
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