| Summary: | 碩士 === 國立臺灣大學 === 應用力學研究所 === 92 === This thesis takes advantage of dielectric property of material, where a dielectrophoretic (DEP) force is induced in a sinusoidally time-variant electric field to achieve cell-plasma separation and cell manipulation. Dielectrophoretic theory is based on the distinct dielectric and conductive properties of cell and medium. This distinction will physically induce a directional force depending on frequency, spatial electric strength, and spatial electric phase distribution.
Through fabrication of MEMS, devices miniaturized are to increase the influence of DEP force on separation and manipulation of cells. Additionally, with the aid of numerical simulation of electric field and cell trajectory, more effective devices are designed. The use of bio-compatible material polydimethysilloxane (PDMS) proved ease of fabrication and integration [1].
Types of cell-plasma separator tested various electrode design include stair, inclined, and gradient confuguration, and 3D channel assisting design. For cell manipulation, traveling wave, cell concentrator, and cell portioning devices are all tested and their performance quantified.
Results show successful separation of red blood cell (RBC) and plasma vis DEP. for a wide range of electrode geometry configurations. Traveling wave DEP, however, was more difficult to implement. Manipulation of RBC proved viable using the non-uniform E-field at the tip of multi-electrode design.
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