| Summary: | 碩士 === 國立臺灣大學 === 食品科技研究所 === 92 === Malignant tumor is ranked top among the ten major causes of death with an increasing mortality year by year. Dendritic cell (DC) is a potent antigen presenting cell (APC) and is capable of uptake and processing foreign antigens, such as bacteria and tumor cells, and presents specific peptides of antigen to major histocompability complex (MHC). As a consequence, activation of T cells occurs and leads to strong adaptive immune response. Such characteristics of DC enable it to be focused recently on cancer therapy.
In this study, to study the growth inhibition of activated mixed lymphocytes in presence with various levels of cold water extracts from PO on CT26 cells. Mouse DCs were cultured from bone marrow and then activated and matured by incubation for 1 day with LPS or CT26 cell lysates, which were prepared by repeated freezing and thawing. The mature DCs were then co-cultured with mixed lymphocytes from mouse splenocytes at a ratio of 1/10 to enhance the proliferation and specific cytotoxicity of mixed lymphocytes (containing T-cells). Subsequently, the activated mixed lymphocytes were co-cultured with CT26 cells (at a ratio of 1/20), in the presence or absence of PO water extracts, to observe the inhibition on CT26 cells.
Results showed that mature DCs were effective in proliferating and enhancing the cytotoxic specificity of mixed lymphocytes. PO cold water extracts exhibited CT26 cell inhibition on growth, while stimulating the proliferation of mixed lymphocytes, as detected by a MTT assay. However, PO cold water extracts showed anti-proliferation on activated mixed lymphocytes by about 20 %. The activated mixed lymphocytes displayed remarkable inhibition of CT26 cells by about 80 %; however, in the presence (200 - 600 μg/mL) of PO cold water extracts, they showed much stronger inhibition on CT26 cells by about 90 %, revealing the activation of DCs and activated mixed lymophocytes by PO cold water extracts.
On the other hands, PO hot water extracts proliferated both CT26 cells and activated mixed lymphocytes. Although PO cold water extracts showed adverse results on CT26 cells and mixed lymphocytes, they were effective in inhibiting CT26 cell growth by increased cytotoxity of T cells as a result of the co-cultivation with PO extracts. Reasons for proliferating or anti-proliferating the activated mixed lymphocytes could be due to the difference in compositions between PO cold and hot water extracts.
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