Expression of diamine oxidase-like genes in mouse reproduction tracts

• Main Authors: Chun-Hui Lin, 林純慧
• Other Authors: Yee-Hsiung Chen
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/75546086017490100600

碩士 === 國立臺灣大學 === 生化科學研究所 === 92 === Four Abp1-like genes, including Abp1、Svs I and two hypothetic genes Loc243375、Loc243376 were identified in mouse chromosome 6B2.3. A phylogenic analysis suggested that these genes may evolve from an ancestral gene. The results of RT-PCR with cDNA from sexual glands revealed the exclusive expression of Abp1 in female and SvsI、Loc243375、Loc243376 in male, in particular seminal vesicle. Further, Loc243375 and Loc243376 were slightly expressed while Svs I was greatly expressed in seminal vesicle. The uterine expression of Abp1 was correlated with the level of ovarian steroid hormones during estrous cycle. Mouse SVS I in seminal vesicle and amiloride binding protein (Abp) in uterus share 56 % identity in their primary structure. We prepared the antibody specific to each of these two proteins. Abp was immunolocalized in the luminal epithelium and glandular epithelium of uterus. This in line with the immunodetection of Abp in the uterine fluid demonstrated the secretion of Abp from the uterine epithelium. The result of diagonal electrophoresis manifested the homodimmer formation of Abp by disulfide linkage. Abp showed diamine oxidase (DAO) enzyme activity. Both the protein level and the enzyme activity reached climax in estrus stage during estrous cycle. On the other hand, SVS I showed no DAO activity. Instead, SVS I forms complexes with other components in seminal vesicle fluid by disulfide bond. SVS I may become a better substrate of transglutaminase as a result of the intermolecular disulfide bonds among SVS I、SVS II、and SVS III in the mouse seminal vesicle secretion. Apparently, duplication of the Abp1-like genes results in various functions in order to meet the physiological requirement.