Development and Applications of Evanescent Planar Wave in the Detection of DNA Microarray

• Main Authors: Peng-chen Huang, 黃鵬丞
• Other Authors: Pei-Kuen Wei
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/33654800796216939117

碩士 === 國立臺灣海洋大學 === 光電科學研究所 === 92 === Biotechnology is a key industry in the twenty-first century. Many kinds of the biochips were developed to expedite the speed of bio-interaction, increase the throughput or detection sensitivity. Among those biochips, the DNA microarray chip is developed most quickly and can be widely used. In this thesis, I will develop new detection technology for the reading of DNA microarray chip. The goals are to develop a high-throughput microarray reader with high sensitivity and large area detection capabilities. I adopt the concept of “Planar Waveguide Biosensor”, which was developed by the Zeptosens Company. I use the glass slide, a common substrate for the DNA microarray chip, as the waveguide layer. Three different waveguide coupling technologies are developed and tested their performances. They are tapered coupling, transmission grating coupling and reflection grating coupling. For the tapered coupling, a tapered angle between 22o and 30o needs to be made for the requirements of total reflection and uniform guiding in the glass slide. From the experimental results, the tapered coupling can provide large detection area and can detect Cy3 molecules of 10pM concentration (0.06 fluors/μm2) in 5 minutes. For the grating coupling, a PDMS layer was used as the grating layer. The PDMS has very good adhesion to glass and can be used repeatedly. In the setup of transmission grating coupling, due to the destruction of PDMS grating, the performance is not good. For the reflection grating coupling, we can achieve the sensitivity of 1pM concentration of Cy3 (0.006 fluors/μm2) in 5 minutes reading time on 3cm×2cm detection area.