Study of DNA detection using silver enhancement

• Main Authors: Yen-Chung Lin, 林彥彰
• Other Authors: 黃男農
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/98295246201969468050

碩士 === 國立臺灣海洋大學 === 機械與輪機工程學系 === 92 === In this study, the silver enhancement technique coupled with the electric current measurement is employed for DNA detection. First, aluminum electrodes with a gap of 140μm are facilitated on the surfaces of glass substrates using MEMS technology. The surface of glass is modified to improve the immobilization of probe DNA. The target DNA labeled with biotin is hybridized with probe DNA and conjoined with Streptavidin-gold . The silver is deposited on the nano-gold particles, which increases the size of metal particles and forms a circuit between electrodes. The effects of silver solution (ratio, volume and concentration), reaction conditions (temperature, duration and number of repetition) and the reaction duration of fixer on the silver enhancement are examined. Optimal operation conditions, which increase the signal-to-noise ratio, are obtained. Target DNA with a concentration of 0.1μM can be identified.