| Summary: | 碩士 === 國立臺灣海洋大學 === 機械與輪機工程學系 === 92 === In this study, the silver enhancement technique coupled with the
electric current measurement is employed for DNA detection. First,
aluminum electrodes with a gap of 140μm are facilitated on the surfaces
of glass substrates using MEMS technology. The surface of glass is
modified to improve the immobilization of probe DNA. The target DNA
labeled with biotin is hybridized with probe DNA and conjoined with
Streptavidin-gold . The silver is deposited on the nano-gold particles, which
increases the size of metal particles and forms a circuit between electrodes.
The effects of silver solution (ratio, volume and concentration),
reaction conditions (temperature, duration and number of repetition) and
the reaction duration of fixer on the silver enhancement are examined.
Optimal operation conditions, which increase the signal-to-noise ratio, are
obtained. Target DNA with a concentration of 0.1μM can be identified.
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