| Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 92 === Abstract
Lactic acid bacteria (LAB) were used to ferment four dehulled adlay yogurts. Four dehulled adlay are: Taichung No. 1 (Tc1), Taichung Shuenyu No. 4 (Ts4), Thailand imported (Th), and Laos imported (La). The 3 g, 4 g, and 5 g four dehulled adlay yogurts that pH value under 4.6, titrable acid for 0.78-0.87%, and LAB counts above 8 log CFU/g, by Lactobacillus (Lb.) plantarum BCRC10069 (1%) and BCRC12250 (1%) fermented at 37oC for 24 hr. Four dehulled adlay yogurt cold water extracts (12.5 mg/mL) were proceeded three antioxidant test. The ability for scavenging DPPH free radical of 5 g Taichung No. 1 dehulled adlay yogurt (Tc1-f) was better, as 10.20%. As to the ability of chelating on Fe (II) ion, the results indicated that four dehulled adlay yogurts performed above 60% chelating effect on Fe (II) ion. In the antioxidant effect as measurement on inhibition of hemoglobin-induced linoleic acid oxidation, the results showed four dehulled adlay yogurts as 11.96-104.81% antioxidant activity. Four dehulled adlay yogurts that pH value from original 4.54-4.63 falls to 4.32-4.42, titrable acidity from 0.77-0.78% increases to 0.95-1.02%, LAB counts still maintain above 8 log CFU/g during 4oC for 14 days. The ability of scavenging DPPH free radical and chelating on Fe (II) ion with store time increase, the antioxidant activity was gradually decreased. S. typhimurium TA100 was used in the Ames test, and the results showed 3-5 g four dehulled adlay yogurt cold water extracts were antimutagenicity ability to 4NQO and B[a]P. In the immuno-cells physiology activity experiment, both Thailand (Th-f) and Laos’s dehulled adlay yogurts (La-f) were growth-promotion ability to human hybridoma HB4C5 cell, and to the human acute monocyte THP-1 cell, four dehulled adlay yogurts were growth-promotion ability. SD rats experience result discovers that yogurt diet had lower relative hepatic weight and body weight when compared with those animals fed with the control diet. Th-f diet had lower in liver cholesterol and triglyceride content. Dehulled adlay yogurt diet induced a decrease liver AST and ALT levels, and increase fecal content of rats. In addition, rats fed the Taichung Shuenyu No. 4 dehulled adlay yogurt (Ts4-f) diet had higher in fecal cholesterol and triglyceride content. A significant increase in cecal contents and a significant decrease in fecal pH value were observed in rats fed with the yogurt diet. It was interesting to note that rats fed with the dehulled adlay yogurt diet had elevated microbial count on fecal Lactobacillus (increased from 7.37 to 7.72 log CFU/g), Bifidobacterium (increased from 7.41 to 8.78 log CFU/g), and total anaerobes (increased from 8.72 to 9.85 log CFU/g), as depressed Clostridium (C.) perfringens count (decreased from 5.39 to 3.50 log CFU/g).
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