Studies on the etiology of winter mass mortality in cultured small abalone （Haliotis diversicolar supertexta）and it’s preliminary gill-cell culture

• Main Authors: Wei-Yi Wu, 吳韋毅
• Other Authors: Ping-Chung Liu
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/27950637639473675461

碩士 === 國立臺灣海洋大學 === 水產養殖學系 === 92 === Abstract Outbreak of mass mortality among cultured small abalone Haliotis diversicolor supertexta occurred in January and February of 2003 in North-east coast of Taiwan. The outbreak occurred in a short period of time with high mortality rate, The moribund animals manifested less elasticity and darkness of the mantle. These gross signs were different from that caused by bacteria. The injection of tissue filtrates from moribund abalone into healthy abalones caused 100％mortality within 48~72 hours post-challenge. Virus-like particles（90-100 nm）were observed in the gill tissues of moribund animals by using TEM microscope technology. The virus-like particle was suggested as the causative agentof the disease outbreak. For further etiology study, the establishment of possible cell lines was conducted. The preliminary results revealed that gill tissue were easier than other organ to avoid contamination during the trial of establishment using L-15 (eibovitz’s L-15 medium). After a seriers of trials, the optimal condition for gill tissue culture was the use of L-15 midium supplemented with 10％ fetal calf serum and cultured at 20℃. The subculture are now continued for 5 generation. The majority of the tissues cultured were epithelial-like cells with 20 chromosomes stained. Atpresent, no CPE was observable in gill-tissues cultured when infected with tissue-filtrates previously preserved at -196℃.