| Summary: | 碩士 === 國立臺灣海洋大學 === 水產養殖學系 === 92 === Abstract
This study was evaluate the effect of Vitamin C and β-glucan on non-specific immune response of cobia (Rachycentron canadum) by oral administration. We measured the non-specific immune immunological parameters such as the superoxide anion (O2-) production and the enzyme activites of glutamate oxaloacetate transaminse (GOT)、glutamate pyruvate transaminse (GPT) and superoxide dismutase (SOD).
The result showed that significant (P＜0.05) increase of intrahaemocytic O2- than intact groups was occur in cobia fed with diets containing different dose of vitamin C and β-glucan after using nitrblue tetrazolium (NBT) reduction assay. Moreover, the O2- production was highest at day 2-4. However, on day 8 the O2- production was lowest. The SOD enzyme activity of cobia at different sampling time was variation. A significant (P＜0.05) increase of enzyme activity of superoxide dismutase was generated in cobia fed with diet containing different dose of Vitamin C and β-glucan from 4-8 day. However, in contrast with O2- production, we found that increased O2- production was observed from 2-4 day. The enzyme activity of GOT in serum of cobia fed with diet containing different dose of vitamin C were lower than intact group (P＜0.05). This result indicated that Vitamin C were helpful to wound heating of cobia.
The results also showed that the cobia had higher immune response when Vitamin C was used together with β-glucan. And the best administration dose is 4g/kg Vitamin and 8g/kg β-glucan.
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