| Summary: | 碩士 === 國立海洋大學 === 水產養殖學系 === 92 === Antimicrobial peptides have been recognized to play important roles in the innate immune system of invertebrate. Most of antimicrobial peptides contain positive charge and low molecular weight, which have the activity against Gram-positive and Gram-negative bacteria, fungi, viruses, and parasites. We had constructed a plasmid containing 6 fragments of antimicrobial peptide gene, pET28KM132-6F. pET28KM132-6F had been transformed into E. coli Rosetta cell. The recombinant antimicrobial peptide KMTrx-6F had been induced by IPTG or lactose, purified by Mono-S and Superdex-75 column, and analyzed by radial diffusion assay. The molecular weight of recombinant antimicrobial peptide KMTrx-6F is about 28 kDa. The minimum inhibitory concentration of recombinant antimicrobial peptide KMTrx-6F is 0.5 μg/ml. The recombinant antimicrobial peptide KMTrx-6F is heat-stable. The antimicrobial activity of recombinant antimicrobial peptide KMTrx-6F is increased when it is dissolved in 0.1% acetic acid. The recombinant antimicrobial peptide KMTrx-6F has the antibacterial ability against Gram-negative aquatic pathogenic bacteria.
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