Design Inhibitors of Shiga-like Toxin and Gene Carrier with Gold Nanoparticle

• Main Authors: Chen, Yi-Chen, 陳怡辰
• Other Authors: Chen, Chia-Chun
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/72500054415104841621

碩士 === 國立臺灣師範大學 === 化學研究所 === 92 === Escherichia coli O157:H7 produce the B subunit of Shiga-like toxin. Infection with E.coli O157:H7 in human results initially in diarrhea in the victims, leading to colitis and progresses further to hemolytic uremic syndrome which is a direct result of SLT-induced kidney damage, a major cause of acute renal failurein children. Shiga-like toxins are AB5 toxins with an enzymatically active A-component and a cell binding B component. The B polypeptide forms a pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3). Shiga-like toxins then enter the cells by receptor-mediated endocytosis. This thesis includes that the purified method of Shiga-like toxin Ι B subunit and explore the multivalent interactions between Shiga-like toxin Ι B subunit and Pk antigen analogues studied using surface plasmon resonance (SPR). We design different size of gold nanoparticles encapsulated by Pk antigen analogues having various lengths of alkyl chains as inhibitors and compare their inhibitory effect.