Biochemical analysis of porcine articular cartilage

• Main Authors: Chia-Han Lin, 林家漢
• Other Authors: Yen-Chung Chang
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/d3f38s

碩士 === 國立清華大學 === 分子醫學研究所 === 92 === We use porcine articular cartilage as an experimental model to extract proteins and glycosaminoglycans to optimize the conditions for extraction of proteins and GAGs from cartilage and the procedures for carrying out biochemical analyses of the extractable materials. Currently, we can extract sufficiently large amount of proteins after treating the lyophilized cartilage slices with guanidine HCl at 60℃, and we have conducted proteomic analysis of these extractable proteins. The work still in progress include: to optimize the extract conditions for glycosaminoglycans and to establish the methodology for glycosaminoglycans analysis. We will analyze normal and pathological human cartilage after establishing above methodologies in order to understanding the function of various cartilage components and to apply useful information for the tissue engineers to develop artificial cartilage.