| Summary: | 碩士 === 國立清華大學 === 生物資訊與結構生物研究所 === 92 === Cobra phospholipase A2, a α-helix acidic polypeptide, is known to hydrolyze membrane and involve some cell signaling pathways. Herein, we demonstrate that the major cobra phospholipase A2 ( Naja atra ), By the combined polarized attenuated total reflection infrared spectroscopy and computer simulation studies, Naja atra phospholipase A2 is shown to peripherally bind to multilayers in a similar edgewise manner with a tilted angle of 160° between theα-helix plane of the Cobra phospholipase A2 molecule and the normal of the membrane surface.
Qualitatively speaking, theα-helix plane of the Cobra phospholipase A2 molecule with (90°,60°) orientation appears to be more perpendicular to the membrane surface than that with (160°, 60°) orientation.
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