| Summary: | 碩士 === 國立中山大學 === 生物科學系研究所 === 92 === SKD1 (suppressor of potassium transport growth defect) belongs to the AAA-ATPase family and is one of the class E VPS (vacuolar protein sorting) proteins. The ATPase activity-deficient form of SKD1 leads the perturbation of mechanism transport through endosomes and lysosomes, however, the molecular mechanism behind the action of SKD1 is poorly understood. In this study, it is identified that VPS4_YEAST (Saccharomyces cerevisiae) is the homology protein of mcSKD1 (Mesembryanthemum crystallinum SKD1) employing sequence profile analysis. The full-length mcSKD1 protein possesses MIT, AAA-ATPase, and NACHT domains of VPS4_YEAST. It is investigated that VPS4_YEAST (NACHT domain) interacts to BRO1_YEAST (HEAT repeat), BRO1_YEAST (HEAT repeat) to AIP1_YEAST (WD40 repeat), AIP1_YEAST (WD40 repeat) to ABP1_YEAST (ADF domain), ABP1_YEAST (ADF domain) to ARP2_YEAST (ACTIN domain), respectively, from yeast two-hybrid system via protein-protein interaction. These proteins employ the function of cytoskeleton structural proteins in cells. Hence, mcSKD1 protein may involve the mechanisms of potassium ion uptake and salt tolerance via the function of cytoskeleton structural proteins.
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