Effect of Sulfated Fructooligosaccharides on the Inhibition of Angiogenesis

碩士 === 國立東華大學 === 生物技術研究所 === 92 === According to previous reports, the sulfated polysaccharides and oligosaccharides can inhibit angiogenesis. Evidences show that angiogenesis is regulated to balance by the interaction between stimulators and inhibitors such as growth factors, cytokines, and proteo...

Full description

Bibliographic Details
Main Authors: Pi-Feng Su, 蘇碧鳳
Other Authors: Tzong-Zeng Wu
Format: Others
Language:zh-TW
Published: 2004
Online Access:http://ndltd.ncl.edu.tw/handle/99707891040906779212
id ndltd-TW-092NDHU5108013
record_format oai_dc
spelling ndltd-TW-092NDHU51080132016-06-17T04:16:06Z http://ndltd.ncl.edu.tw/handle/99707891040906779212 Effect of Sulfated Fructooligosaccharides on the Inhibition of Angiogenesis 磺酸化果寡糖及其抑制血管增生之研究 Pi-Feng Su 蘇碧鳳 碩士 國立東華大學 生物技術研究所 92 According to previous reports, the sulfated polysaccharides and oligosaccharides can inhibit angiogenesis. Evidences show that angiogenesis is regulated to balance by the interaction between stimulators and inhibitors such as growth factors, cytokines, and proteolytic enzymes. In some diseases like cancer, rheumatoid arthritis, and diabetic retinopathy, angiogenesis is out of control in balance condition and causes abnormal angiogenesis. This thesis is to study the inhibition of angiogenesis by sulfated fructooligosaccharides(FOS). First, we used Aspergillus japonicus mycelia to produce FOS and obtained higher purity of FOS by charcoal activated column and ethanol purification process, then carried sulfation processes by replacing the —OH groups in FOS molecules to —OSO3 group. Finally, we examined the sulfated FOS by chick chorioallantoic membrane(CAM) assay to see whether it had anti- angiogenesis effectiveness or not. Experiment results showed that the conversion of sugars to FOS by Aspergillus japonicus mycelia could over 50%; the purity of FOS by charcoal activated column purification could reach 99%. After FOS sulfation, its color changed from transparent to yellowish-brown. If the further sulfated process ongoing, the color would become dark brown. The result of HPLC analysis showed that the nature of FOS after sulfation was changed. From the result of chick CAM assay, we found that there had obvious inhibition of angiogenesis while the dosage of sulfated FOS was over 1mg. We speculated that sulfated FOS might interact with bFGF and VEGF, which caused the proliferation and migration of endothelial cells, therefore inhibited the angiogenesis. Tzong-Zeng Wu 吳宗正 2004 學位論文 ; thesis 95 zh-TW
collection NDLTD
language zh-TW
format Others
sources NDLTD
description 碩士 === 國立東華大學 === 生物技術研究所 === 92 === According to previous reports, the sulfated polysaccharides and oligosaccharides can inhibit angiogenesis. Evidences show that angiogenesis is regulated to balance by the interaction between stimulators and inhibitors such as growth factors, cytokines, and proteolytic enzymes. In some diseases like cancer, rheumatoid arthritis, and diabetic retinopathy, angiogenesis is out of control in balance condition and causes abnormal angiogenesis. This thesis is to study the inhibition of angiogenesis by sulfated fructooligosaccharides(FOS). First, we used Aspergillus japonicus mycelia to produce FOS and obtained higher purity of FOS by charcoal activated column and ethanol purification process, then carried sulfation processes by replacing the —OH groups in FOS molecules to —OSO3 group. Finally, we examined the sulfated FOS by chick chorioallantoic membrane(CAM) assay to see whether it had anti- angiogenesis effectiveness or not. Experiment results showed that the conversion of sugars to FOS by Aspergillus japonicus mycelia could over 50%; the purity of FOS by charcoal activated column purification could reach 99%. After FOS sulfation, its color changed from transparent to yellowish-brown. If the further sulfated process ongoing, the color would become dark brown. The result of HPLC analysis showed that the nature of FOS after sulfation was changed. From the result of chick CAM assay, we found that there had obvious inhibition of angiogenesis while the dosage of sulfated FOS was over 1mg. We speculated that sulfated FOS might interact with bFGF and VEGF, which caused the proliferation and migration of endothelial cells, therefore inhibited the angiogenesis.
author2 Tzong-Zeng Wu
author_facet Tzong-Zeng Wu
Pi-Feng Su
蘇碧鳳
author Pi-Feng Su
蘇碧鳳
spellingShingle Pi-Feng Su
蘇碧鳳
Effect of Sulfated Fructooligosaccharides on the Inhibition of Angiogenesis
author_sort Pi-Feng Su
title Effect of Sulfated Fructooligosaccharides on the Inhibition of Angiogenesis
title_short Effect of Sulfated Fructooligosaccharides on the Inhibition of Angiogenesis
title_full Effect of Sulfated Fructooligosaccharides on the Inhibition of Angiogenesis
title_fullStr Effect of Sulfated Fructooligosaccharides on the Inhibition of Angiogenesis
title_full_unstemmed Effect of Sulfated Fructooligosaccharides on the Inhibition of Angiogenesis
title_sort effect of sulfated fructooligosaccharides on the inhibition of angiogenesis
publishDate 2004
url http://ndltd.ncl.edu.tw/handle/99707891040906779212
work_keys_str_mv AT pifengsu effectofsulfatedfructooligosaccharidesontheinhibitionofangiogenesis
AT sūbìfèng effectofsulfatedfructooligosaccharidesontheinhibitionofangiogenesis
AT pifengsu huángsuānhuàguǒguǎtángjíqíyìzhìxuèguǎnzēngshēngzhīyánjiū
AT sūbìfèng huángsuānhuàguǒguǎtángjíqíyìzhìxuèguǎnzēngshēngzhīyánjiū
_version_ 1718307268206788608