Virtual 2D Electrophoresis System: 2D Gunner System

• Main Authors: Meng-Hsien Kao, 高孟賢
• Other Authors: Huey-jenn Chiang
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/95057041841505350579

碩士 === 國立東華大學 === 生物技術研究所 === 92 === Proteomics research aims at the functional analysis of gene products, such as large-scale identification, localization and interaction studies of proteins. For studying the expressed proteins, 2D-Gel Electrophoresis and Mass Spectrometry are two major techniques. In this work, we developed a virtual 2D-Gel Electrophoresis system, 2D Gunner, using the sequence information of total potential ORFs to calculate the theoretical pI and MW for simulating the 2D-Gel Electrophoresis patterns. The researcher could preliminary obtain the information of interested protein spots, including protein sequences as well as some basic properties, prior to characterization by mass spectrometry. Compared with the theoretical pI/MW values provided by ExPASy, the correlations are 0.99/ 0.99. In the future, we will continue to improve the correctness of this virtual 2D-gel Electrophoresis pattern, to calibrate the patterns with the actual experiment data and to correlate the spots with the protein information.